This phenomenon is common in allergic diseases. However, the immunological mechanisms of albumin sensitization have not yet been investigated in detail although this will be important for the development of allergen-specific prevention and allergen-specific immunotherapy (AIT) strategies for allergy to albumin. Cross-reactivity refers to an antigenantibody reaction that occurs when a specific antibody reacts with antigen (s) other than just the primary sensitizing antigen. In the latter scenario few differences in the sequences of Fel d 2 and Can f 3 as compared to HSA would be responsible for the development of selective T cell and B cell responses towards Fel d 2 as well as Can f 3. This tag system depends on specific detection of flag fusion proteins and no cross-reactivity to cellular proteins. If the first assumption is correct it should be possible to detect HSA-specific T cell responses and HSA-containing immune complexes in sensitized patients. An example of helpful cross-reactivity is in heterophile antibody tests, which detect Epstein-Barr virus using antibodies with specificity for other antigens. One possibility is failed development of immune tolerance in albumin allergic patients whereas the other possibility is highly selective immune tolerance to HSA but not to Fel d 2 and Can f 3. Here we discuss two possibilities how IgE sensitization to Fel d 2 and Can f 3 may develop. The cells were labeled with Myc Tag Monoclonal Antibody (Product MA1-980-1MG) at 1:100 dilution and Histone H3 antibody (Product 711055) at 1:200 dilution in 0. Given the high degree of sequence identity between the latter two allergens and HSA one would expect that immunological tolerance would prohibit IgE sensitization to Fel d 2 and Can f 3. Sequence identity of cat albumin (Fel d 2) and dog albumin (Can f 3) and HSA is 82% and 80%, respectively. It is astonishing that allergic patients can develop specific IgE responses against animal albumins because these proteins exhibit a more than 70% sequence identity to human serum albumin (HSA) which is the most abundant protein in the blood of the human body. Approximately 20-30% of cat and dog allergic patients show IgE reactivity and mount IgE-mediated allergic reactions to cat and dog albumin. You just subscribed to receive the final version of the articleĪlbumins from animals are highly cross-reactive allergens for patients suffering from immunoglobulin E (IgE)-mediated allergy. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |